Figure 3
Scatterplot and matching heatmap of circRNA expression between (A) control and clone 1 and (B) control and clone 9. The values of X and Y axes in the scatterplot are the normalized signal values of the samples (log2 scaled) or the averaged normalized signal values of groups of samples (log2 scaled). The green lines in the scatterplot indicate FC. Heatmap reflects changes in expression using unsupervised clustering analysis (euclidean distance measure and the ‘average’ agglomeration method) (n = 3). Red indicates higher levels of expression, while blue indicates lower levels. circRNAs chosen for validation are outlined in smaller heat maps showing the top five up and down regulated circRNAs in clone 1 vs. control (C) and clone 9 vs. control (D)(n = 3). Green indicated reduced expression, with red indicating increased expression. (E) Venn diagram displaying differentially expressed and overlapping circRNAs between clone 1 and clone 9 vs. control.
