Table 1 Kinetic parameters of IQF peptide processing. Substrate R1IQF: GLPVFAEFLPLFSKFGSRMHIL(K-Abz)STRN↓GLPSY(3NO2).

From: A suite of kinetically superior AEP ligases can cyclise an intrinsically disordered protein

Enzyme

kcat (s−1) ±sem

Km (µM) ±sem

kcat/Km (M−1s−1)

OaAEP1b

0.99 ± 0.06

146 ± 16

6774

OaAEP3

0.61 ± 0.07

1.86 ± 0.8

329,982

OaAEP4

~0.76

<0.77

~983,865

OaAEP5

0.59 ± 0.01

2.4 ± 0.43

247,117

Back to article page