Table 7 Primers used in the study

From: High frequency of the exoU+/exoS+ genotype associated with multidrug-resistant “high-risk clones” of Pseudomonas aeruginosa clinical isolates from Peruvian hospitals

Amplified product

Primers

Sequence (5′ → 3′)

Amplicon size (bp)

Annealing Temperature

Ref.

Type III secretion system

   exoU

exoU - F

ATG CAT ATC CAA TCG TTG

2000

58 °C

65

exoU - R

TCA TGT GAA CTC CTT ATT

   exoS

exoS - F

GCG AGG TCA GCA GAG TAT CG

118

58 °C

65

exoS - R

TTC GGC GTC ACT GTG GAT

   exoT

exoT - F

AAT CGC CGT CCA ACT GCA TGC G

152

58 °C

65

exoT - R

TGT TCG CCG AGG TAC TGC TC

   exoY

exoY - F

CGG ATT CTA TGG CAG GGA GG

289

58 °C

65

exoY - R

GCC CTT GAT GCA CTC GAC CA

Quinolone resistance determining region of gyrA/parC and efflux pumps regulators

   gyrA

gyrA - F

TTA TGC CAT GAG CGA GCT GGG CAA CGA CT

364

57 °C

61

gyrA - R

AAC CGT TGA CCA GCA GGT TGG GAA TCT T

   parC

parC - F

ATG AGC GAA CTG GGG CTG GAT

208

57 °C

61

parC - R

ATG GCG GCG AAG GAC TTG GGA

   mexZ

mexZ - F

CCA GCA GGA ATA GGG CGA CCA GGG C

1059

64 °C

61

mexZ - R

CAG CGT GGA GAT CGA AGG CAG CCG G

   nfxB

nfxB - F

CGC CCC GAT CCT TCC TAT T

924

64 °C

61

nfxB - R

ACG AGC GTC ACG GTC CTT T

Metallo-β-lactamases

   imp

IMP - F

GGA ATA GAG TGG CTT AAY TCT C

188

55 °C

66

IMP - R

CCA AAC YAC TAS GTT ATC T

   vim

VIM - F

GAT GGT GTT TGG TCG CAT A

390

55 °C

66

VIM - R

CGA ATG CGC AGC ACC AG

   gim

GIM - F

TCG ACA CAC CTT GGT CTG AA

477

55 °C

66

GIM - R

AAC TTC CAA CTT TGC CAT GC

   spm

SPM - F

AAA ATC TGG GTA CGC AAA CG

271

55 °C

66

SPM - R

ACA TTA TCC GCT GGA ACA GG

   sim

SIM - F

TAC AAG GGA TTC GGC ATC G

570

55 °C

66

SIM - R

TAA TGG CCT GTT CCC ATG TG

Serine-carbapenemases

   kpc

KPC - F

GTA TCG CCG TCT AGT TCT GC

636

55 °C

67

KPC - R

GGT CGT GTT TCC CTT TAG CC

   imi

IMI - F

ATA GCC ATC CTT GTT TAG CTC

818

55 °C

68

IMI - R

TCT GCG ATT ACT TTA TCC TC

   ges

GES - F

GTT TTG CAA TGT GCT CAA CG

371

55 °C

68

GES - R

TGC CAT AGC AAT AGG CGT AG

Multi-locus sequence typing

   acsA

acsa - F

ACC TGG TGT ACG CCT CGC TGA C

524

60 °C

45

acsA - R

AGG TTG CCG AGG TTG TCC AC

   aroE

aroE - F

TGG GGC TAT GAC TGG AAA CC

1053

60 °C

45

aroE - R

TAA CCC GGT TTT GTG ATT CCT ACA

   guaA

guaA - F

CGG CCT CGA CGT GTG GAT GA

673

60 °C

45

guaA - R

GAC GTT GTG GTG CGA CTT GA

   mutL

mutL - F

AGA AGA CCG AGT TCG ACC AT

705

60 °C

45

mutL - R

GGG TAT AGG CGG AAT AGC C

This Study

   nuoD

nuoD - F

GCT TCA AGC CGG AAG ACT GG

438

60 °C

This Study

nuoD - R

TGG CGG TCG GTG AAG GTG AA

45

   ppsA

ppsA - F

GGT CGC TCG GTC AAG GTA GTG G

556

60 °C

45

ppsA - R

GTA TCG CCT TCG GCA CAG GA

   trpE

trpE - F

TTC AAC TTC GGC GAC TTC CA

603

60 °C

45

trpE - R

CCC GGC GCT TGT TGA TGG TT

  1. bp: basepair; Ref: Reference; F: Forward; R: Reverse.
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