Figure 1 | Scientific Reports

Figure 1

From: Preferential phosphatidylinositol 5-phosphate binding contributes to a destabilization of the VHS domain structure of Tom1

Figure 1

(a) Modular organization of Tom1 with the domain boundaries labeled. (b) SDS-PAGE showing the expression and purification of recombinant GST-Tom1 VHS and untagged Tom1 VHS. Lane 1, cell extract before IPTG induction; lanes 2–4, cell extracts after 1, 2, and 4 h incubation with IPTG, respectively; lane 6, protein soluble cell fraction; lane 7, purified GST-Tom1 VHS bound to glutathione beads; lane 8, purified untagged Tom1 VHS. (c) Size-exclusion chromatography showing the elution of Tom1 VHS. Inset: fractions containing purified Tom1 VHS. (d) Electrospray ionization-MS analysis of recombinant Tom1 VHS. (e) DLS analysis of purified Tom1 VHS at 25 °C.

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