Figure 3 | Scientific Reports

Figure 3

From: Preferential phosphatidylinositol 5-phosphate binding contributes to a destabilization of the VHS domain structure of Tom1

Figure 3

PtdIns5P-dependent destabilization of the Tom1 VHS domain. (a) SDS-PAGE showing the trypsin limited proteolysis of Tom1 VHS in the absence (i) and presence of PtdIns5P (ii), PtdCho (iii), and ubiquitin (iv). (b) Far-UV CD spectra of Tom1 VHS in the absence (black) and presence of PtdIns5P (red) or PtdCho (green) under nonreducing conditions at 37 °C. The loss of signal at 218–222 nm is boxed.

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