Figure 4 | Scientific Reports

Figure 4

From: Preferential phosphatidylinositol 5-phosphate binding contributes to a destabilization of the VHS domain structure of Tom1

Figure 4

Conformational changes of Tom1 VHS associated to PtdIns5P and ubiquitin binding under nonreducing conditions. (a) Intrinsic tryptophan fluorescence of Tom1 VHS in the absence and presence of increasing concentrations of PtdIns5P. Inset: Extent of PtdIns5P-dependent quenching of Tom1 VHS signal. (b) Intrinsic tryptophan fluorescence of Tom1 VHS in the absence and presence of increasing concentrations of ubiquitin. Inset: Extent of ubiquitin-dependent quenching of Tom1 VHS signal.

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