Figure 2 | Scientific Reports

Figure 2

From: Interaction of PIAS1 with PRRS virus nucleocapsid protein mediates NF-κB activation and triggers proinflammatory mediators during viral infection

Figure 2

Identification of NF-κB activating proteins of PRRSV. NF-κB luciferase activities of nonstructural proteins and structural proteins. HeLa cells grown in 12-well plates were transfected with pNF-κB-Luciferase (0.5 μg), pRL-TK (0.05 μg), and each (0.5 μg) of indicated PRRSV genes for 24 h. Cells were treated or mock-treated with TNF-α (20 ng/ml) for 6 h, and cell lysates were prepared in passive lysis buffer for luciferase assays as described in Materials and Methods. Relative luciferase activities were obtained by normalizing the firefly luciferase to Renilla luciferase activities. Values of the relative luciferase activity in the pXJ41 control group were set as 1 and the values for individual viral proteins were normalized using that of the pXJ41 control. Error bars, mean ± standard deviation (s.d.). (n = 3). ***P < 0.001.

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