Figure 7
CP26 reduces DENV and ZIKV replications in Huh-7 cells. (A) The maximal non-toxic dose of CP26 was determined using Cell Proliferation Reagent WST-1. (B) CP26 at 200 nM reduced the number of DENV2 viral particles 48 hrs post-infection, as revealed by immunostaining for the flavivirus group antigen. (C) Huh-7 cells were seeded in 12-well plates and infected with DENV2 for 48 hrs at an MOI of 0.1. Virus-infected cells were treated with increasing concentrations of CP26, and viral RNA levels were measured by qPCR. (D) Decreased virus production in cells infected by four serotypes of DENV and four strains of ZIKV 48 hrs post-treatment with 200 nM CP26 (ANOVA with Tukey post-test, n.s.: no significant difference, **p < 0.01 vs. DMSO control, ***p < 0.001 vs. DMSO control).
