Figure 4
From: Concentric lamellae – novel microanatomical structures in the articular calcified cartilage of mice
The appearance of concentric lamellae around chondrocytes in the ACC of aged BALB/c AKU mice. (a) A chondrocyte partially surrounded by concentric lamella, yet not completely enclosed in the ACC (x6000). Inset: Location of chondrocyte in the ACC, showing apparent ‘opening’ of the tidemark (arrowed) resulting in the cell becoming engulfed by the ACC (x2500). (b) A chondrocyte almost completely surrounded by lamellae, progressing deeper into the ACC (x6000). Inset: Location of chondrocyte in the ACC, showing apparent ‘closing’ of the tidemark (arrowed) resulting in the cell becoming completely embedded in the ACC (x2500). (c) A chondrocyte surrounded by numerous concentric lamellae (arrowed) in a periodic-like manner (x8200). (d) Concentric lamellae surrounding a chondrocyte deep in the ACC, in a periodic manner (arrowed) identical to what was seen in (c) (x8200). Tissues fixed in (a,b) PBFS, (c,d) glutaraldehyde. Ages = (a,b) 60 wks, (c,d) 54.4 wks. Scale = (a,b) 5 µm, (c,d) 2 µm.
