Figure 1 | Scientific Reports

Figure 1

From: The interleukin-33-mediated inhibition of expression of two key genes implicated in atherosclerosis in human macrophages requires MAP kinase, phosphoinositide 3-kinase and nuclear factor-κB signaling pathways

Figure 1

The effect of IL-33 on MCP-1 and ICAM-1 expression in primary macrophages. RT-qPCR for MCP-1 (A) and ICAM-1 (B) was carried out using cDNA against RNA from primary HMDMs that were incubated for 12 h in the presence of vehicle (−) or 25 ng/ml of IL-33 (+). The mRNA levels were calculated using the comparative Ct method and normalized to the housekeeping gene, GAPDH, with values from vehicle-treated control cells given an arbitrary value of 1. Data represents mean ± SEM from four independent experiments. Statistical analysis was carried out using an unpaired Student’s t test (*p ≤ 0.05).

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