Figure 2
From: Colorectal cancer cells respond differentially to autophagy inhibition in vivo
Colorectal cancer cells feature different autophagic flux. mCherry:GFP:Atg8 analysis of autophagic flux in the various CRC cell lines. (A) Representative images of mCherry:GFP:Atg8 transfected cells under full media or glucose starvation. (B) Per cell average percentage of autophagosomes and autolysosomes (n = 20 to 65 cells per condition from 3 independent experiments); mean ± SEM. (C) Representative images of TEM analysis in full media or under glucose starvation. Pink highlighted objects represent autophagosomes while yellow highlighted objects are lysosomes/autolysosomes. (D) Number of autophagic vesicles per cell averaged from twelve cells. Only double-membrane vesicles were counted as autophagosomes and electron dense vesicular structures were counted as autolysosomes; mean ± SEM. Statistical analysis was performed using (B) Two-way ANOVA followed by Tukey’s multiple comparison test or (D) One-way ANOVA followed by Kruskal-Wallis multiple comparisons test.
