Figure 5
From: Colorectal cancer cells respond differentially to autophagy inhibition in vivo
AKT expression is increased in autophagy-inhibited SW480 cells. A variety of signaling kinases were differentially phosphorylated upon autophagy inhibition. (A) Phosphoarray normalized ratios from three independent ATG5- and RAB21-tumors normalized to pooled scrambled control tumors. Each bar represents duplicates for each tumor. Statistical significance was determined using one sample T-test. (B) Immunoblot analysis of total AKT1/2/3 and tubulin from multiple SW480 tumors extracted five days post-implantation in CAMs. (C) Ratio of AKT1/2/3 integrated densities to Tubulin for all tumors; mean ± SEM (n ≥ 22). (D) Immunoblot analysis of various phosphorylation sites on AKT, SGK1S422 and total AKT1/2/3 and Tubulin expression from multiple independent SW480 tumors extracted five days post-implantation in CAMs. (E) Ratio of phosphorylated AKT or SGK1 integrated densities to Tubulin for all tumors; mean ± SEM (n ≥ 4). Statistical significance was determined using One-way ANOVA followed by Kruskal-Wallis multiple comparisons test. Full-length blots are presented in Supplementary Fig. 6.
