Figure 7
From: Colorectal cancer cells respond differentially to autophagy inhibition in vivo
AKT and mTORC inhibition blocks SW480 hyperproliferation observed in ATG5- and RAB21-depleted cells. Efficiency validation of AKT and mTOR inhibitors. (A) Immunoblot analysis of phosphorylated PRAS40T246 upon addition of increasing concentrations of MK2206. (B) Immunoblot analysis of phosphorylated S6K1T389 upon addition of increasing concentrations of Rapamycin and Torin 2. Treatments were performed for 48 hours. (C) Quantification of SW480 tumor growth treated with different inhibitors. The dotplot graph depicts tumor sizes 5 days post-implantation in CAMs with drug treatments in at least 3 independent experiments; mean ± SEM. (D) Quantification of SW480 tumor growth treated with different inhibitors and transfected with scrambled, ATG5 or RAB21 siRNA. The dotplot graph depicts tumor sizes 5 days post-implantation in CAMs with drug treatments in at least 3 independent experiments; mean ± SEM. Statistical significance was determined using One-way ANOVA followed by Kruskal-Wallis multiple comparisons test. Full-length blots are presented in Supplementary Fig. 6.
