Figure 2

Germination of fungal spores following plasma treatment. (a) Number of germinated A. oryzae spores after treatment with nitrogen (N₂) gas (control) and plasma in PBS or PDB. (b) Relative spore germination percentage following plasma treatment compared to that of the control (N₂ gas treatment): (number of germinated spores treated with plasma or gas/number of germinated spores treated with gas only) × 100. (c) FACS analysis of spore size. Spores treated with plasma in PBS and PDB for 2 min and 5 min, respectively, were analyzed. Black and red lines represent N₂ gas (control) and plasma treatment, respectively. Each value was averaged from a total of 24 replicates in (a,b) and FACS analysis was repeated once in (c). *p < 0.05.