Figure 5
From: Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma
Analysis of A. oryzae spore membrane potential by DiBAC4(3) staining. Histograms show the proportion of fungal spores with different fluorescence intensities following DiBAC4 staining (3). Fluorescence indicates that the cell membrane is depolarized. (a) The proportion of fungal spores exhibiting different fluorescence intensities following plasma treatment for 2 min in PBS and 5 min in PDB. Black and red lines represent spores treated with nitrogen (N2) gas (control) and plasma, respectively. (b) The proportion of fungal spores exhibiting different fluorescence intensities following treatment with the indicated concentrations of SNP or H2O2 dissolved in PBS and PDB. Treatment time was 2 min for PBS and 5 min for PDB solutions. Black and red lines represent spores treated without and with H2O2 and SNP, respectively.
