Figure 7
From: Aspergillus oryzae spore germination is enhanced by non-thermal atmospheric pressure plasma
Western blot analysis of MAPK phosphorylation following plasma treatment. Total proteins were extracted from fungal spores incubated for 4 h following plasma treatment (2 min in PBS and 5 min in PDB). N2; treatment with only N2 gas, N2P; treatment with N2 and plasma. Arrows indicate protein bands corresponding to each MAP kinase (hogA or mpkA) with or without phosphorylation and β-actin (reference protein). The phosphorylation and protein levels of each MAP kinase were detected using following antibodies: hogA (expected molecular weight 41.8 kDa); anti-phospho p38 (phosphorylation) and anti-Hog1 (protein), mpkA (expected molecular weight 47.8 kDa); anti-phospho p44/42 (phosphorylation) and anti-p44/42 (protein).
