Figure 1 | Scientific Reports

Figure 1

From: Paxillin S273 Phosphorylation Regulates Adhesion Dynamics and Cell Migration through a Common Protein Complex with PAK1 and βPIX

Figure 1

Paxillin S273 phosphorylation combined with PAK1 kinase activity, βPIX GEF activity, βPIX binding to PAK1 and phosphatase activity regulate CHO-K1 cell migration. (A) Representative images of CHO-K1 cells expressing paxillin-WT-EGFP (left), -S273A (middle), and -S273D (right). Scale bars for images are 5 μm. (B) Cell migration speed expressing paxillin-WT, –S273A, -S273D with expression of PAK1-WT, -KD,-CA or βPIX-WT, -ΔSH3, -LL. (C) Rose plots of 8 representative cell migration paths of cells expressing paxillin-WT, -S273D, -S273A fused with EGFP. (D) Migration speed of paxillin-WT-EGFP cells treated with 10 μM IPA-3 (PAK1 kinase inhibitor) or 50 nM okadaic acid (PP2a phosphatase inhibitor). Error bars represent SEM. Two stars (**) corresponds to p < 0.001, three stars ***p < 0.0001. n = 90 cells, 30 cells from three independent experiments.

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