Figure 4 | Scientific Reports

Figure 4

From: Paxillin S273 Phosphorylation Regulates Adhesion Dynamics and Cell Migration through a Common Protein Complex with PAK1 and βPIX

Figure 4

PAK1 and βPIX do not visibly co-localize with paxillin but are enriched in adhesions. (A) Laser scanning confocal images of cells co-expressing paxillin-WT-EGFP with transiently transfected βPIX-WT or PAK1-WT mCherry fusions. Scale bar is 10 μm. (B) Intensity analysis was performed on adhesions and adjacent non-adhesion areas. Scale bar is 5 μm. The inset illustrates how the analysis was achieved (cyan vs yellow ROIs). Distribution of (C) paxillin-WT-EGFP, (D) PAK1-WT-mCherry and (E) βPIX-WT-mCherry intensity levels binned according to percentage enrichment in adhesions and adjacent non-adhesion areas. Positive values on the histogram (dark blue or dark red) represent enrichment higher in the adhesions and negative numbers denote a higher enrichment in non-adhesion areas (light blue or light red). Paxillin was always enriched in the adhesion versus the non-adhesion areas. n = 130–140 adhesions and non-adhesion regions from at least 10–15 cells.

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