Figure 6 | Scientific Reports

Figure 6

From: Paxillin S273 Phosphorylation Regulates Adhesion Dynamics and Cell Migration through a Common Protein Complex with PAK1 and βPIX

Figure 6

Paxillin phosphorylation state at S273 influences the degree of interaction between paxillin and PAK1 or paxillin and βPIX at sites of adhesion. (A) Spatial protein co-dynamic binding maps across cells co-expressing PAK-WT or βPIX-WT mCherry fusions and paxillin-WT, -S273A, or -S273D EGFP fusions. (B) Spatial protein co-dynamic maps across cells expressing paxillin-WT-EGFP and PAK1-WT-mCherry before, 30 and 60 minutes after treatment with 2.5 μM IPA-3 (PAK1 inhibitor) or 50 nM Okadaic acid (PP2A phosphatase inhibitor). Scale bars are 5 μm. Red pixels indicate where the two proteins are co-binding.

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