Figure 7

Paxillin co-binding with PAK1 or βPIX occurs at new dynamics edges of adhesions. Spatial protein binding maps of cells co-expressing paxillin-WT-EGFP and (A) PAK1-WT-mCherry or (B) βPIX-WT-mCherry. Red pixels indicated areas of co-binding. Co-binding occurs proximal to protrusive cell edges (cyan lines) and distal from retractive cell edges (orange lines) as shown in enlarged areas indicated by white boxes. Cells were imaged over time and as protrusive and retractive regions changed, so did the location of co-binding. Scale bars are 5 μm.