Figure 4
From: Short-term CFTR inhibition reduces islet area in C57BL/6 mice
Islet size and composition. (a) Representative images of pancreatic islets from mice treated with vehicle control, streptozotocin, CFTRinh172, or a combination of the two and stained for insulin (green), glucagon (red), and the nuclear stain DAPI (blue). (b) Islet area (µm2), (c) beta cell area [insulin positive area] (µm2), (d) non-beta cell area [non-insulin positive area] (µm2), (e) beta cell number as a percentage of total islet cells [insulin positive cells/DAPI count] and (f) alpha cell number as a percentage of total islet cells [glucagon positive cells/DAPI count]. Data are presented as mean ± SEM (n = 5 mice) and calculated from two sections of tissue per animal taken at least 200 µm apart. A minimum of 100 islets were counted from a total of 5 animals (at least 20 islets per animal). Each data point on the graphs represents one islet. **p < 0.01 and ***p < 0.001 compared to controls animals (One-way ANOVA).
