Figure 5
From: Short-term CFTR inhibition reduces islet area in C57BL/6 mice
Pdx-1 expression. (a) Representative images of pancreatic islets from mice treated with vehicle control, streptozotocin, CFTRinh172, or a combination of the two and stained for insulin (green), Pdx-1 (red), and the nuclear stain DAPI (blue). (b) Number of Pdx-1 positive cells as a percentage of beta cells (insulin positive cells). Data are presented as mean ± SEM (n = 5 mice). A minimum of 50 islets were counted from a total of 5 animals (at least 10 islets per animal). Each data point on the graphs represents one islet. ***p < 0.001 compared to control animals (One-way ANOVA). (c) Relative PDX1 mRNA expression in the isolated islets of control animals and those treated with CFTRinh172 alone. Data are presented as mean ± SEM (n = 8).
