Figure 4
From: Low immunogenic endothelial cells endothelialize the Left Ventricular Assist Device
HLA class I-silenced ECs respond to external stimuli. RT-PCR analysis of the endothelial phenotype (FLT-1), endothelial specific activation (ICAM-1, VCAM-1, E-Selectin) and thrombogenic state markers (tissue factor, thrombomodulin) of NC, shNS and shβ2m on SICs, with and without TNFα-stimulation indicated a stable endothelial phenotype and a physiological response to TNFα-stimulation, which is not affected by the cell-to-SIC contact, or by silencing HLA-class I expression. The paired two-tailed t-test and the one-way ANOVA analysis were used to determine statistically significant differences (p < 0.05) indicated by the asterisks (*). Statistically insignificant results are marked as “ns”. The results are expressed as group means ± standard deviation (n = 3).
