Figure 5
From: Low immunogenic endothelial cells endothelialize the Left Ventricular Assist Device
Lower thrombogenicity of endothelialized SIC compared to native SIC. Native or endothelialized SIC with HLA class I-expressing or silenced ECs were exposed to platelets, which were activated with ADP and thrombin for 1 h. Platelets were labeled with Allophycoyanin conjugated anti-CD61 antibody (magenta) and ECs expressed GFP as reporter gene (green). Thrombus formation was analyzed by fluorescence microscopy, indicating that (a) native SIC was more thrombogenic than (b) SIC endothelialized with HLA class I-expressing ECs or (c) SIC endothelialized with HLA class I-silenced ECs. (d) Mean fluorescence intensities are depicted in the bar graph. The paired two-tailed t-test was used to determine statistically significant differences (p < 0.05) indicated by the asterisks (*). The results are expressed as mean ± standard deviation (n = 3).
