Figure 6
From: Low immunogenic endothelial cells endothelialize the Left Ventricular Assist Device
Regulation of endothelial HLA class I silencing by Doxycycline. ECs expressing shβ2m and the trans-activator tTR-KRAB were cultured in the presence or absence of 100 ng/ml doxycycline and after removal of doxycycline. Levels of β2-microglobulin transcripts (a) measured by real-time PCR were higher in non-modified ECs, transduced ECs in the absence of doxycycline (shβ2m – Dox) or after withdrawal of doxycycline (shβ2m Dox removed). (b) These results were confirmed at protein level as detected by flow cytometric analysis of HLA class I expression. The bar graphs represent mean values and standard deviations (n = 3). (c) Representative overlay of HLA class I expression in presence and absence of doxycycline.
