Figure 8
From: Low immunogenic endothelial cells endothelialize the Left Ventricular Assist Device
HLA class I-silenced EC on SIC are protected against antibody-mediated complement-dependent cytotoxicity. ECs typed for HLA-A*02 were used to coat SIC. NC, shNS or shβ2m ECs on SIC were exposed to specific anti-HLA-A*02 or non-specific anti-HLA-A*23 antibodies and complement. Levels of cell lysis, indicated by LDH activity, were measured by detecting optical densities (OD) at 450 nm. ECs expressing shβ2m showed significantly lower levels of LDH activity in comparison with NC and shNS, indicating that HLA class I-silenced EC on SIC are protected against antibody-mediated complement-dependent cytotoxicity. ANOVA was used to determine statistically significant differences indicated by the asterisks (*p < 0.05, **p < 0.01). The results are presented as mean ± standard deviation (n = 3).
