Table 1 Real time PCR details of the three mRNA analyzed, NCBI reference is the sequence used for the primers design, sequences of both the forward and reverse primers and their annealing temperature (aT).
From: Aortic remodelling induced by obstructive apneas is normalized with mesenchymal stem cells infusion
mRNA | NCBI reference | Forward primer | Reverse Primer | aT |
---|---|---|---|---|
eNOS | NM_021838 | GGATCCAGTGGGGGAAACTG | TGGCTGAACGAAGATTGCCT | 60 |
iNOS | NM_012611.3 | GGCAGGATTCAGTGGTCCAA | GAACATTTCTGATGCAGTGCTACA | 60 |
PPIB | NM_022536.2 | AGCGCAATATGAAGGTGCTCT | CTTATCGTTGGCCACGGAGG | 60 |