Figure 1

Visual representation of the experimental workflow used to study complexome changes. Schematic representation of the workflow used to study changes in the protein complexome. Protein extracts were prepared from fully expanded Arabidopsis thaliana leaves under native conditions and separated by native-PAGE. The proteins were subsequently digested using a modified in-gel digestion procedure, and the peptides desalted using C18 columns. The obtained peptides were analysed by LC-MS/MS and the raw spectral information exported to MaxQuant for protein identification and peak based label-free quantification. All downstream data analyses were performed using R statistical software.