Figure 4

Visualization by Fluorescent In Situ hybridization (FISH) of Streptomyces sp. and K. pneumoniae within (a) an adult female scabies mite, (b) mite feces within epidermal burrows and (c) an egg. An adjacent section (i) was stained with Hematoxylin for orientation. The universal EUB338 probe (red, ii) was labelled with CalFlour590 while Streptomyces sp. (fuchsia, iii) and K. pneumoniae probes (green, iv) were both labelled with Cy5. Positive staining is visible in the gut area of the mite indicated as ‘g’ in section ‘i’ of panel ‘a’, and in the feces indicated as ‘f’ of panel (b).