Figure 7
From: Stress-induced phospho-ubiquitin formation causes parkin degradation
Phospho-Ub-induced parkin degradation is proteasomal. (A,B) Differentiated PC12 cells were co-treated with either 10 μM CCCP (A) or 200 μM L-DOPA (B) and 100 nM epoxomicin for 12 (A) or 24 (B) hours before being harvested for Western immunoblotting. Representative Western immunoblots and quantification of parkin levels are shown. * in blots marks a known non-specific band. Error bars show SEM from N = 5 (A) and 8 (B) independent experiments; *p ≤ 0.05, ***p ≤ 0.001, by paired t-test with Holm correction for multiple comparisons. Images of blots have been cropped; uncropped images are shown in Supplementary Fig. 12. In B, left and right images show non-adjacent bands on the same blot.
