Table 1 List of Primers used for genotyping and RT-qPCR.

LRT Forward

TGT GTG CCC GTC TGT TGT GT

59.4

LRT Reverse

GAG TCC TGC GTC GAG AGA GC

63.5

LRT Probe

FAM -CAG TGG CGC CCG AAC AGG GA- TAM

65.5

mTitin forward

AAA ACG AGC AGT GAC GTG AGC

59.8

mTitin reverse

TTC AGT CAT GCT GCT AGC GC

59.4

mouse beta forward

GCT GCT GGT TGT CTA CCC TTG

61.8

human gamma forward

GTG GAA GAT GCT GGA GGA GAA

60.3

human beta forward

CGT GCT GGT CTG TGT GCT G

61

mouse alpha forward

AGC TGA AGC CCT GGA AAG GAT

59.8

human alpha forward

CAG ACT CAG AGA GAA CCC ACC AT

62.4

mouse beta reverse

CCC ATG ATA GCA GAG GCA GAG

61.8

Human gamma reverse

TGC CAA AGC TGT CAA AGA ACC T

58.4

human beta reverse

CTT GTG GGC CAG GGC ATT AG

61.4

mouse alpha reverse

GCC GTG GCT TAC ATC AAA GTG

59.8

human alpha reverse

GCC TCC GCA CCA TAC TCG

60.5

mouse beta probe

FAM -CCA GCG GTA CTT TGA TAG CTT TGG AGA CC –TAM

68.1

human gamma probe

FAM - AGG CTC CTG GTT GTC TAC CCA TGG ACC -TAM

69.5

human beta probe

FAM - CCC ATC ACT TTG GCA AAG AAT TCA CCC -TAM

65

mouse alpha probe

FAM -TGC TAG CTT CCC CAC CAC CAA GAC C -TAM

67.9

human alpha probe

FAM -TGC TGT CTC CTG CCG ACA AGA CCA A -TAM

66.3

mTitin probe

FAM - TGC ACG GAA GCG TCT CGT CTC AGT C -TAM

67.9

Mouse β KI reverse

GTCAGAAGCAAATGTGAGGAGCA

62.5

β KI forward

TTGAGCAATGTGGACAGAGAAGG

62.4

B383 KI reverse

GTTTAGCCAGGGACCGTTTCA

62