Figure 3 | Scientific Reports

Figure 3

From: Identification of a factor controlling lysosomal homeostasis using a novel lysosomal trafficking probe

Figure 3

Drug screening of the lysosomal pathway by the lysosomal-METRIQ probe. Evaluation of the effects of known cancer-related inhibitors against the membrane-trafficking pathway and lysosomal activity. HeLa Tet-On cells expressing the lysosomal-METRIQ probe were cultured in regular medium with Dox for 48 h and treated with each drug from the SCADS inhibitor library for 12 h prior to analysis. The red/green fluorescence ratio was measured using flow cytometry. Kenpaullone obtained from ALEXIS and Calbio chem is indicated with * and **, respectively. Candidate membrane trafficking or lysosomal activity downregulators are coloured in blue. Candidate lysosomal biogenesis upregulators are coloured in red.

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