Figure 4

Force-feeding MM cancer cell exosomes back to MM cells leads to cancer cell death. (A) PKH67 labeled exosomes from Hmeso cells added to Hmeso cells show uptake/interaction of exosomes with target cancer cells. (B) Phase contrast images of Hmeso cells after addition of three different concentrations of Hmeso exosomes (concentrations based on volume of cell media collected from) or Mock (ExoQuick-TC), and (C) MTS proliferation assay of force feeding MM cancer exosomes, n = 6. Similar experiments with H2373 cells by (D) phase imaging and (E) MTS proliferation assay, as well as with H2595 MM cells by (F) phase imaging and (G) MTS proliferation assay, n = 6. Force feeding Hmeso exosomes to Hmeso MM cells (H) significantly reduced protein levels of CCND1 as analyzed by Western blot analysis. Western blot images shown are representative images and quantitation graphs of (I) CCND1 are combined quantitation of 3 repeated experiments. N = 3, Phase contrast images were taken with 40× objective lens, scale bar = 100 µm, mean ± SEM, *p ≤ 0.05 as compared to control and ^† p ≤ 0.05 as compared to Mock by 1-way ANOVA.