Figure 5

miR-16 overexpression in Hmeso cells leads to decreased MM cancer cell proliferation and protein abundance of CCND1 and BCL2. (A) Cellular levels of miR-16 increased after transfection, whereas, contradictorily, (B) miR-16 levels in exosomes significantly decreased upon transfection of miR-16 into Hmeso cells. n = 3. The target proteins of miR-16 (C) (immunoblot), (D) CCND1 and (E) BCL2 were reduced after miR-16 transfection. Transfection of miR-16 also resulted in (F) significantly reduced proliferation of MM cells by MTS, and increased cell death by cisplatin (n = 6), along with attenuated capabilities of (G) migration (n = 3) and (H) invasion (n = 3). (I) miR-16 transfection had no effect on secreted exosome numbers as assessed by NTA. All miRNA qPCR data is normalized to synthetic spike-in control cel-miR-39-3p, which was added to all exosome or cell isolates prior to RNA isolation. Mean + SEM, *p ≤ 0.05 as compared to control and ^†p ≤ 0.05 as compared to cisplatin by 1-way ANOVA.