Figure 6

HuR is involved in the exosomal secretion of miR-16 from MM cells. (A) Transfection of siHuR significantly reduced the protein abundance of HuR as analyzed by Western blot analysis (B), and led to significantly increased cellular expression of miR-16 and (C) significantly decreased expression of miR-16 in exosomes. (D) Transfection of miR-16 mimic similarly induced significant reductions in HuR protein levels as analyzed by Western blot analysis (using the same immunoblot as Fig. 5, therefore B-actin is the same). n = 3. mean + SEM, *p ≤ 0.05 as compared to control by 1-way ANOVA.