Figure 1
From: RORα suppresses interleukin-6-mediated hepatic acute phase response
Administration of JC1-40 reduces the DEN-induced APR in the liver. JC1-40, 20 mg/kg BW/day, was administered orally for 3 days to the mice and then the mice were i.p. injected with 100 mg/kg BW DEN for 2 days before sacrificed. (a) Serum GPT and GOT activity were measured. (b) Serum levels of IL-6, TNFα, and IL-1β were determined by ELISA. (c) TUNEL staining of liver sections were conducted. TUNEL positive cells were counted using image J (top). Immunohistochemistry staining of γH2AX in the liver sections. The percentage of γH2AX positive nuclei was measured by Image J (bottom). (d) Immunohistochemistry staining of CXCL1 and LCN2 in the liver sections. The stained area were measured by Image J. The analysis of stainings were conducted from at least 6 images per tissues. Scale bars: 100 μm. *P < 0.05, and **P < 0.01; #P < 0.05, and ##P < 0.01 (n = 3-5) for (a–d). The data represent mean ± SD.
