Figure 3
From: RORα suppresses interleukin-6-mediated hepatic acute phase response
RORα represses IL-6-induced activation of STAT3. (a) JC1-40, 20 mg/kg BW/day, was administered orally for 3 days to the mice and then the mice were i.p. injected with 100 mg/kg BW DEN for 2 days before sacrificed. The protein levels of pSTAT3 in liver tissues were analyzed by western blotting. (b) Primary mouse hepatocytes were infected by Ad-GFP or Ad-RORα for 24 h and then treated with 10 ng/ml IL-6 for an additional 24 h (left). Or primary mouse hepatocytes were treated with 50 μM JC1-40 for 8 h in the presence or absence of 10 ng/ml IL-6 for 8 h (right). The protein levels of pSTAT3 and RORα in hepatocytes were analyzed by western blotting. (c) HepG2 cells were transfected with the STAT3RE-Luc reporter. After 24 h of transfection, cells were infected by Ad-GFP or Ad-RORα for 24 h in the presence or absence of 1 ng/ml IL-6 for 6 h (left), or treated with 50 μM JC1-40 for 24 h in the presence or absence of 1 ng/ml IL-6 for 6 h (right). Luciferase activities were normalized by corresponding β-galactosidase activity. *P < 0.05; #P < 0.05 (n = 3). (d) Primary mouse hepatocytes were transfected with si-GFP or si-RORα for 24 h and then treated with 10 ng/ml IL-6 for an additional 24 h. The protein levels of pSTAT3 and RORα in hepatocytes were analyzed by western blotting. The data represent mean ± SD. Representatives of at least three independent experiments with similar results are shown.
