Figure 4
From: RORα suppresses interleukin-6-mediated hepatic acute phase response
RORα represses expression of IL-6Rα. (a) A scheme for regulation of intracellular STAT3 signaling (left). Primary mouse hepatocytes were infected by Ad-GFP or Ad-RORα for 24 h. The mRNA levels of the indicated genes were measured by qRT-PCR (right). *P < 0.05 (n = 3). (b) Primary mouse were infected by Ad-GFP or Ad-RORα for 24 h. The protein levels of the indicated genes were analyzed by western blotting. (c) Primary mouse hepatocytes were treated with 50 μM JC1-40 for 24 h. The hepatic mRNA levels of IL-6Rα were analyzed by qRT-PCR (left). The protein levels of IL-6Rα and RORα in hepatocytes were analyzed by western blotting (right). *P < 0.05 (n = 3). (d) Primary mouse hepatocytes were transfected with si-GFP or si-RORα for 48 h. The protein levels of IL-6Rα and RORα in hepatocytes were analyzed by western blotting. (e) JC1-40, 20 mg/kg BW/day, was administered orally for 3 days to the mice and then the mice were i.p. injected with 100 mg/kg BW DEN for 2 days before sacrificed. Immunohistochemistry staining of IL-6Rα in the liver sections. The stained area were measured by Image J. Scale bars: 200 μm. **P < 0.01 and ##P < 0.01 (n = 5). The data represent mean ± SD.
