Figure 5
From: RORα suppresses interleukin-6-mediated hepatic acute phase response
RORα suppresses transcription of IL-6Rα via direct binding to an intronic region of the mouse Il6ra gene. (a) ChIP-seq reads of RORα and liver input control in the genome loci of Il6ra are shown in ChIP-seq track. ChIP-seq peaks were visualized in Integrative Genomics Viewer (Broad Institute). (b) Primary mouse hepatocytes were infected by Ad-GFP or Ad-RORα for 24 h. DNA fragments that contain RORα enrichment region indicated by public ChIP-seq datasets were immunoprecipitated with the anti-RORα or anti-AcH3K9. (c) HepG2 cells were transfected with the Signal 3 RORE-luc reporter, and were infected by Ad-GFP or Ad-RORα for 24 h. Luciferase activities were normalized by corresponding β-galactosidase activity. *P < 0.05; #P < 0.05 (n = 3). The data represent mean ± SD. (d) Schematic model for the suppressive mechanism of RORα in hepatic IL-6-STAT3-mediated APR.
