Figure 2 | Scientific Reports

Figure 2

From: Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis

Figure 2

Characterization of EV. (a) Average size density histogram of five canine MSC-derived EV samples evaluated in NTA light scatter mode; error bars represent ±1 standard error. (b) Appearance of CD9-biotin/Qd655-SAV labeled EV in light scatter (top) and fluorescence (bottom) modes on NTA. (c) Western blots of canine EV and parent cells from three different cell lines demonstrating enrichment for CD9 (top, 19 kDa) and TSG101 (bottom, 49 kDa) in only the EV samples. (d) Transmission electron micrograph of canine EV labeled with CD9-biotin/streptavidin-gold; gold particles appear black, all scale bars are set to 200 nm. (e) Atomic force microscopy of canine EV labeled with CD9-biotin/Qd655-SAV showing a 2.7 μm-wide field.

Back to article page