Figure 6 | Scientific Reports

Figure 6

From: Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis

Figure 6

HPLC depletion of free Qd655. (a) Chromatogram generated by the HPLC DAD at 220 nm and FLD at 655 nm highlighting the early immunolabeled vesicle fraction (F1), later free Qd655-SAV fraction (F2), and a late peak representing small molecular weight proteins. (b) Particle size distribution assessed using light scatter mode (LSM, red line) and fluorescent mode (FM, blue line) in samples prior to separation (pre-HPLC) and within each collected fraction (F1, F2); error bars represent ±1 standard error. (c) Assessment of FM:LSM and SA percentages for particles ranging in size from 50–200 nm pre- and post-HPLC separation. (d) Percentage of the total sample particle count determined in FM comprised of particles <50 nm in size pre- and post-HPLC separation.

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