Table 1 Particle sizes calculated by NTA in fluorescent (FM) and light scatter (LSM) modes.

From: Improved methods for fluorescent labeling and detection of single extracellular vesicles using nanoparticle tracking analysis

 

Dog 1

Dog 2

Dog 3

Dog 4

Dog 5

HEK-293

NTA calculation of mean particle size

CD9

FM

128.3 ± 3.2

130.5 ± 3.8

134.4 ± 15.6

165.9 ± 8.3

144.3 ± 7.3

167.5 ± 2.4

LSM

154.7 ± 5.2

156.3 ± 1.9

151.6 ± 7.9

187.0 ± 6.3

149.1 ± 4.7

157.5 ± 4.3

Isotype

FM

31.7 ± 1.8

37.2 ± 3.6

29.6 ± 7.2

37.9 ± 2.9

27.1 ± 1.1

20.1 ± 2.0

LSM

134.2 ± 3.8

126.7 ± 3.7

136.0 ± 4.2

160.5 ± 6.1

130.3 ± 2.5

106.0 ± 5.2

NTA calculation of mode particle size

CD9

FM

91.0 ± 8.0

90.7 ± 4.0

100.8 ± 4.5

104.1 ± 12.8

102.7 ± 9.6

112.0 ± 15.7

LSM

104.0 ± 6.6

126.5 ± 6.4

118.8 ± 10.8

108.1 ± 8.1

101.7 ± 7.0

118.6 ± 3.9

Isotype

FM

25.5 ± 0.3

26.0 ± 1.5

26.0 ± 4.1

25.2 ± 0.3

24.4 ± 0.2

20.6 ± 1.3

LSM

92.4 ± 2.2

96.4 ± 3.2

114.5 ± 5.9

123.6 ± 12.2

121.4 ± 12.6

64.6 ± 0.8

  1. Size characteristics of immunolabeled EV are reported, including mean and mode particle sizes ± standard error.