Figure 3 | Scientific Reports

Figure 3

From: Antiretroviral therapy partially improves the abnormalities of dendritic cells and lymphoid and myeloid regulatory populations in recently infected HIV patients

Figure 3

Proportions of myeloid-derived suppressor cells (MDSCs) and intracellular expression of interleukin 10 by them in healthy controls (n = 20) and in untreated patients with HIV infection: recent (n = 10) and chronic (n = 10). (A) MDSCs (percentage of leukocytes), quantified using anti-lineage (Cocktail Lin clones CD3-SK3, CD14-Mφ9, CD16-3G8, CD19-SJ25L1, CD20-L27,CD56-NCAM16.2.), anti-HLA-DR, anti-CD11b and anti-CD33 antibodies (BD Biosciences). (B) Intracellular expression of interleukin 10 (percentage of MDSCs expressing interleukin 10), determined using specific antibodies (anti-IL-10, clone JES3-19F1, BD Biosciences) after LPS (1 μg/ml) (Sigma) stimulation for 5 hours. For each graphic, flow cytometry data for representative cases of a healthy control and a patient with untreated chronic HIV infection with undetectable HIV load are shown. A grey line in (B) figure represents isotype controls, used to confirm specificity of staining and to discriminate background staining. Data are provided as median, interquartile values and range.

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