Table 1 Statistical analysis of clock and phagocytosis gene mRNA rhythmicity in ARPE-19 dispersed cells and monolayers.

From: Rev-Erbα and Photoreceptor Outer Segments modulate the Circadian Clock in Retinal Pigment Epithelial Cells

Non-linear regression analysis					1-way ANOVA
Gene	P value	Period (h)	Acrophase (h)	Amplitude	P value	F value
Dispersed ARPE-19 cells
Clock genes
ARNTL	0.0003	21.10 ± 0.98	11.01 ± 1.04	0.45 ± 0.09	<0.0001	18.11
CLOCK	0.1493	22.24 ± 2.06	12.61 ± 2.14	0.18 ± 0.08	0.0002	4.07
CRY1	0.0432	28.00 ± 2.84	27.14 ± 1.46	0.21 ± 0.07	<0.0001	4.99
CRY2	0.0072	28.00 ± 2.30	25.90 ± 1.20	0.39 ± 0.11	0.0056	2.73
PER1	0.0007	28.00 ± 1.92	22.40 ± 1.09	0.23 ± 0.05	<0.0001	5.09
PER2	<0.0001	26.47 ± 1.33	21.79 ± 0.87	0.47 ± 0.09	0.0140	2.39
REV-ERBα	<0.0001	22.86 ± 0.77	17.64 ± 0.60	0.53 ± 0.07	<0.0001	6.82
Phagocytosis genes
GAS6	0.0699	20.00 ± 1.38	7.11 ± 1.86	0.17 ± 0.06	0.0064	2.68
ITGB5	0.0021	20.00 ± 0.96	10.46 ± 1.14	0.27 ± 0.06	<0.0001	4.84
LAMP1	0.0265	28.00 ± 2.70	24.24 ± 1.44	0.28 ± 0.09	0.0108	2.48
MFGE8	0.0112	28.00 ± 2.40	26.39 ± 1.24	0.18 ± 0.05	0.0002	4.05
PROS1	0.0029	28.00 ± 2.10	0.62 ± 2.36	0.42 ± 0.11	<0.0001	12.69
PTK2	0.6378	28.00 ± 6.44	2.38 ± 7.23	0.07 ± 0.06	0.0007	3.55
ARPE-19 monolayers
Clock genes
ARNTL	0.0234	20.48 ± 1.57	12.63 ± 1.64	0.36 ± 0.11	0.0066	2.60
CLOCK	0.1745	27.94 ± 3.49	10.69 ± 2.55	0.28 ± 0.12	0.3330	1.17
CRY1	0.0187	20.54 ± 1.11	6.36 ± 1.44	0.26 ± 0.08	0.0023	2.97
CRY2	0.1784	26.28 ± 2.64	20.93 ± 1.83	0.24 ± 0.11	0.0485	1.89
PER1	0.0025	26.59 ± 1.78	18.29 ± 1.29	0.53 ± 0.13	0.0349	2.01
PER2	0.0005	21.86 ± 1.07	19.39 ± 0.84	0.85 ± 0.19	0.0611	1.84
REV-ERBα	0.0024	20.84 ± 1.11	14.70 ± 1.12	0.40 ± 0.10	0.0165	2.27
Phagocytosis genes
GAS6	0.5007	28.00 ± 5.11	24.45 ± 2.79	0.12 ± 0.08	0.5375	0.94
ITGB5	0.0295	28.00 ± 2.75	23.07 ± 1.52	0.27 ± 0.09	0.0038	2.87
LAMP1	0.0479	23.61 ± 1.70	11.29 ± 1.73	0.43 ± 0.15	0.1712	1.44
MFGE8	0.1796	28.00 ± 3.42	21.93 ± 2.01	0.21 ± 0.09	0.5430	0.94
PROS1	0.0030	28.00 ± 2.04	19.38 ± 1.35	0.44 ± 0.11	0.0033	2.84
PTK2	0.0219	28.00 ± 2.40	21.23 ± 1.45	0.25 ± 0.08	0.0044	2.74

Data were fitted to the equation y = y₀ + c⋅cos[2π⋅(t − φ)/τ] by non-linear regression. Bold values represent p < 0.05.

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Table 1 Statistical analysis of clock and phagocytosis gene mRNA rhythmicity in ARPE-19 dispersed cells and monolayers.

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