Table 2 Recommendations for tissue and biospecimen collection.
From: Specimen Collection for Translational Studies in Hidradenitis Suppurativa
Recommendations | Overview |
|---|---|
Consents and Protocols | IRB approved consents and protocols Tissue and biospecimens are collected and utilized for treatment recommendations and research |
Tissue collection & processing | Collect → Observe → Proper storage Transport from OR to bench Proper storage of lysate, RNA, OCT, and paraffin samples Cryostat sectioning of tissue for IHC and IF |
Preparation of tissue for protein analysis | Homogenize whole tissue for Western Blot, ELISA, proteomics, etc. |
Preparation of tissue for nucleic acid analysis | DNA/RNA extraction for genetic sequencing and analysis |
Preparation of tissue for cell culture- Primary and immune cell extraction | Extraction of primary cells Cell culture of fibroblasts, keratinocytes, and melanocytes Extraction of immune cells |
Processing of saliva samples | DNA sample preparation |
Blood processing for serum, nucleic acid and immune cell isolation | Collection and separation, isolation, flow cytometry, RNA extraction |
Skin swabs for microbiome studies | Sequencing and analysis for evaluation of microbial composition |
Overview of sample storage | −20 °C, −80 °C, liquid nitrogen options for storage |
