Figure 1 | Scientific Reports

Figure 1

From: Ultrasound delivery of Surface Enhanced InfraRed Absorption active gold-nanoprobes into fibroblast cells: a biological study via Synchrotron-based InfraRed microanalysis at single cell level

Figure 1

Scheme of the protocols employed for the SR-microFTIR analyses. (A) the nanoprobe assembling was obtained by functionalising the AuNPs with the IR marker 4ATP; spectra were acquired after depositing the nanoprobes onto silicon wafers. (B) the internalisation of the nanoprobes within NIH-3T3 cells was triggered by low-intensity 1 MHz US; the exposure setup employed consists of a water filled tank, a transducer positioned at its bottom and a micro-volume cell holder vertically aligned with the transducer; the sonication was performed on a PBS dispersion of ~106 cells. (C) The samples for SR-microFTIR measurements were prepared by separating the cells from the supernatant and depositing them on silicon substrates by physisorption.

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