Table 3 Summary of diagnostic allele frequency for 24 putative diagnostic markers identified by ddRAD and tested by KASP genotyping assays in 91 individuals to distinguish among O. niloticus, O. mossambicus, O. aureus and O. u. hornorum.

Diagnostic for	Frequency of putative diagnostic allele (%)
Diagnostic for	Marker name	Diagnostic SNP	O. niloticus (n = 30)	O. mossambicus (n = 22)	O. aureus (n = 23)	O. u. hornorum (n = 16)
O. niloticus	Oni3057	A	100*	0	0	0
	Oni9497	T	100*	0	0	31
	Oni5782	A	100*	0	0	0
	Oni2675	G	97*	0	0	0
	Oni1276	A	97*	0	0	0
O. mossambicus	Omo2657	A	0	100*	0	0
	Omo10818	C	0	100*	0	100
	Omo3481	C	0	100*	0	100
	Omo3582	A	0	100*	0	100
	Omo4092	C	0	100*	0	100
	Omo7956	T	0	100*	0	100
	Omo2007	A	0	100*	0	100
	Omo10120	A	0	95*	0	0
	Omo8084	G	0	91*	0	56
O. aureus	Oau9418	C	0	0	100*	0
	Oau8029	G	0	0	100*	0
	Oau966	T	0	0	100*	0
	Oau3873	C	0	0	100*	0
	Oau3001	A	3	0	100*	0
	Oau2890	T	12	0	100*	0
	Oau4411	T	5	0	100*	0
O. u. hornorum	Oho4270	A	0	0	0	100*
	Oho10793	G	0	0	0	100*
	Oho10199	A	0	0	0	100*

Refer to Supplementary Table S3 for full genotype data. *Frequencies of the putative diagnostic alleles in the target species.

Quick links

Search