Figure 7
CLIC4 modulates the apical secretion of MMP2 in polarized human RPE monolayers. (A,B) RPE monolayers differentiated from the human embryonic cell line stably expressing Tet-regulated CLIC4-shRNA were analyzed by immunoblotting assays using the indicated antibodies (A; Best1: Bestrophin1) and by bright-field microscopy (B). (C–G) The above described human RPE monolayers, treated without Dox (Ctrl) or with Dox (KD) for 5–9 days, were analyzed by immunoblotting assays (C; Tub: β-tubulin), ZO-1 staining (D), F-actin staining (E), and MMP2 secretion (F,G). In (E), the confocal images sectioned through the apical and basal sides of the cells are shown. The concentration of the MMP2 was obtained from the cells grown on 38 mm2 (E), and 11 mm2 (F) surface areas, respectively. Bars show means ± S.D. N = 3. p, t-test. Scale bars = 30 µm (B,D); 20 µm (E).
