Figure 4
C1q-recruiting bsAbs bind to target cells and mediate cytotoxicity. (a) Growth of P. aeruginosa PAO1 in human blood was determined in the presence of the indicated antibodies and in blood or media (TSB) alone. ****P < 0.0001, two-way ANOVA showing bsAb samples significantly different from the corresponding bivalent antibody. Results are plotted as mean with standard deviation. (b,c) The ability of antibodies to mediate target cell lysis was measured using CDC assays. Increasing concentrations of the indicated antibodies were incubated with 5% NHS and Raji cells (b; human B-cell line) or Jurkat cells expressing human GITR and CD20 (c; human T-cell line) for 2 or 3.5 h respectively. Cell lysis was quantified using the CytoTox-Glo assay which measures the release of proteases from dead cells. Percent cytotoxicity was calculated as the background subtracted experimental signal divided by the background subtracted maximum lysis signal. Results are plotted as mean ± standard deviation.
