Table 2 Binding characteristics of parental bivalent and C1q-targeting bispecific antibodies.
Antibody | Cell type | Maximum binding (RLUs) | EC50 (M) |
|---|---|---|---|
anti-Psl x anti-C1q | Pseudomonas aeruginosa PAO1 | 3.90 × 105 | 4.32 × 10−9 |
anti-Psl bivalent | Pseudomonas aeruginosa PAO1 | 4.77 × 105 | 3.45 × 10−10 |
anti-CD20 x anti-C1q | Raji- human B-cell | 1.64 × 106 | 3.57 × 10−8 |
anti-CD20 parental bivalent | Raji- human B-cell | 1.71 × 106 | 6.03 × 10−9 |
anti-CD20 bivalent, Rituximab | Raji- human B-cell | 1.85 × 106 | 3.14 × 10−9 |
anti-C1q parental bivalent | Raji- human B-cell | No binding | No binding |
anti-GITR × anti-C1q | Jurkat/hGITR/hDC20- human T-cell | 7.67 × 105 | 3.10 × 10−8 |
anti-GITR bivalent | Jurkat/hGITR/hDC20- human T-cell | 8.97 × 105 | 3.07 × 10−9 |
anti-FelD1 isotype control | Jurkat/hGITR/hDC20- human T-cell | No binding | No binding |
